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Pillar 5: The Supplement Protocol | Longevity Protocol
The Vitality Longevity Protocol
Protocol
The Longevity Protocol · Pillar Five
Pillar Five of Five — The Protocol

The SupplementProtocol

Where the science meets practical application. How the ingredients in the Vitality range relate to the four longevity pillars, what doses published research has used, how to evaluate quality and how to build a longevity supplement protocol that is genuinely evidence-based.

15 min read Final pillar Not health claims
The Ingredients

Every ingredient. Every pillar.

The following maps every ingredient in the Vitality range to the longevity pillar it relates to in published research — not as health claims, but as accurate descriptions of the research context of each compound. Each ingredient is described by what published research has explored, the dose used in studies and how it connects to the broader protocol.

Pillars 1, 2 & 3 — NAD+, Sirtuins, Mitochondria
NMN
500–750mg
Nicotinamide Mononucleotide — the most studied NAD+ precursor food supplement ingredient. One step from NAD+ via the salvage pathway. A systematic review of 10 RCTs with 437 participants documented consistent NAD+ elevation. Relevant to Pillar 1 (NAD+ metabolism), Pillar 2 (sirtuin substrate) and Pillar 3 (SIRT3 and mitochondrial function).
NAD+ precursor 30+ human studies >99% β-NMN purity
Pillars 1 & 2 — NAD+ & Sirtuins
Trans-Resveratrol
600mg
Stilbenoid polyphenol studied extensively in relation to SIRT1 activation since landmark 2003 research. The trans-isomer is the biologically relevant form. At 98% trans-isomer purity — the standard used in published research. Combines with NMN to address both sirtuin activator and NAD+ substrate simultaneously.
SIRT1 research 98% trans-isomer 600mg per serving
Pillar 2 — Sirtuins
Pterostilbene
50mg
Stilbenoid polyphenol — a structural analogue of resveratrol with ~4x higher documented oral bioavailability due to two methoxy groups. Studied in the SIRT1 pathway and metabolic research contexts. Found naturally in blueberries. Included in NMN Complete as a higher-bioavailability sirtuin-pathway polyphenol.
~4x bioavailability vs resveratrol SIRT1 pathway
Pillar 1 — NAD+ (Methylation Support)
TMG
750mg
Trimethylglycine — a methyl donor compound. NAD+ metabolism and biosynthesis consumes methyl groups. TMG replenishes methyl groups used in the methylation cycle and is studied in relation to homocysteine metabolism. Found naturally in beetroot. Included at 750mg in NMN Complete to support methylation alongside NMN.
Methyl donor Homocysteine research 750mg per serving
Pillar 1 — NAD+ (CD38 Pathway)
Apigenin
50mg
A botanical flavone found in chamomile, parsley and celery. Studied in relation to CD38 inhibition — CD38 is the NAD+-consuming enzyme documented to increase significantly with age (covered in Pillar 1). Included in NMN Complete to address the CD38 consumption pathway alongside NMN's NAD+ precursor function.
CD38 pathway research Natural flavone 50mg per serving
Pillar 1 — NAD+ (Reduced Precursor)
NMNH
500mg
Dihydronicotinamide Mononucleotide — the reduced form of NMN. A newer NAD+ precursor ingredient with a distinct chemical structure and active ongoing research. Greater than 99% purity, independently tested on every batch. An alternative or complement to NMN for those interested in newer NAD+ precursor research.
Reduced NAD+ precursor >99% purity Emerging research
Pillar 4 — Cellular Stress
AHCC
1000mg
Active Hexose Correlated Compound — a standardised mushroom extract from Lentinula edodes mycelia. Research origin 1989. One of the most extensively researched mushroom-derived supplement ingredients, with published research spanning over three decades in cellular biology contexts. The primary Pillar 4 ingredient in the Vitality range.
Research since 1989 Standardised extract 1000mg per serving
The Vitality Range

The complete product range

Every Vitality Supplements product is UK manufactured and independently tested by an ISO/IEC 17025-accredited laboratory on every production batch. The following shows how each product fits within the five-pillar protocol.

Most Comprehensive
NMN Complete 1350mg
Pillars 1, 2 & 3 — NAD+ · Sirtuins · Mitochondria · CD38 Pathway

The most comprehensive formula in the Vitality range. Four ingredients targeting the NAD+ precursor pathway, sirtuin activation, methylation support and CD38 inhibition simultaneously. For those who want a single formula that addresses the core NAD+ and sirtuin longevity pillars in full.

750mg NMN 750mg TMG 50mg Pterostilbene 50mg Apigenin
Shop NMN Complete →
Best Value · High Resveratrol
NMN + Trans-Resveratrol 1100mg
Pillars 1 & 2 — NAD+ Precursor · Sirtuin Pathway

The classic NMN and resveratrol combination — addressing both the NAD+ precursor pathway and the sirtuin-pathway polyphenol simultaneously. Provides the highest resveratrol dose in the range at 600mg per serving, 98% trans-isomer purity. The starting point for most people new to NAD+ supplementation.

500mg NMN 600mg Trans-Resveratrol 98% trans-isomer
Shop NMN + Resveratrol →
Newer Precursor · Emerging Research
NMNH 500mg
Pillar 1 — NAD+ (Reduced Precursor)

Dihydronicotinamide Mononucleotide — the reduced form of NMN and a newer NAD+ precursor ingredient with active ongoing research. For those interested in exploring newer NAD+ precursor compounds alongside or instead of NMN. Greater than 99% purity, independently tested on every batch.

500mg NMNH >99% purity Reduced form
Shop NMNH 500mg →
Pillar 4 · Research Since 1989
AHCC 1000mg
Pillar 4 — Cellular Stress & Inflammaging

The primary Pillar 4 product in the Vitality range. AHCC is a standardised mushroom extract with one of the most extensive published research bases of any supplement ingredient in the cellular biology context — over three decades of published research since 1989. Complements the NAD+ and sirtuin-focused pillars by addressing cellular stress biology.

1000mg AHCC Standardised extract Research since 1989
Shop AHCC 1000mg →
How to Build It

Building an evidence-based longevity protocol

An evidence-based longevity supplement protocol is not about taking as many supplements as possible. It is about choosing the right ingredients, in the right doses, from the right sources — and integrating them with the lifestyle factors that the published research most consistently identifies as relevant to longevity biology.

1
Start with the most-researched NAD+ precursor
NMN has the largest recent published human research base of any NAD+ precursor — a systematic review of 10 RCTs with 437 participants. Start here before exploring newer compounds. Dose used in published research: 250–900mg per day. Vitality provides 500mg (NMN + Resveratrol) and 750mg (NMN Complete) per serving.
2
Address the sirtuin pathway
Sirtuins require NAD+ to function — but sirtuin-pathway polyphenols like resveratrol and pterostilbene have been studied as SIRT1 activators. Addressing both the substrate (NAD+ via NMN) and the activation signal (resveratrol or pterostilbene) is the research rationale for the NMN + resveratrol combination. Choose resveratrol for largest research base, pterostilbene for higher bioavailability.
3
Consider methylation support
NAD+ metabolism consumes methyl groups. TMG (Trimethylglycine) is a methyl donor studied in relation to homocysteine metabolism and methylation support. Included at 750mg in NMN Complete. Alternatively, ensure adequate dietary intake of methyl-donor foods — beetroot, eggs, leafy greens.
4
Address cellular stress biology
The four NAD+/sirtuin/mitochondria pillars address one cluster of longevity biology. Cellular stress — inflammaging, oxidative stress, senescent cell burden — is a separate but related cluster. AHCC is the primary Pillar 4 ingredient in the Vitality range, with over three decades of published research.
5
Prioritise lifestyle — supplements are supplementary
The published research most consistently identifies exercise, sleep, caloric moderation and stress management as the most impactful longevity interventions. Supplements are not a substitute. Exercise stimulates mitochondrial biogenesis. Sleep is when NAD+-dependent repair processes are most active. A supplement protocol built on poor lifestyle foundations has a limited ceiling.
6
Consult a healthcare professional
All food supplements should be discussed with a qualified healthcare professional before starting — particularly if you are pregnant, breastfeeding, on medication or have a pre-existing condition. This protocol is educational — not medical advice. Your GP or a registered nutritional therapist can help contextualise it to your specific circumstances.
The best longevity supplement protocol is built on a foundation of sleep, exercise and diet — not on top of a deficit in them.
Quality Criteria

How to evaluate any supplement

The longevity supplement market contains products of highly variable quality. The following five criteria — applied to any NMN or longevity supplement — will separate genuinely high-quality products from those that are not.

  • 01
    Independent batch testing by ISO/IEC 17025-accredited laboratory
    This is the gold standard. An ISO/IEC 17025-accredited laboratory is independently assessed for technical competence. Testing should happen on every production batch — not just once for a launch certificate. Ask for the Certificate of Analysis for the specific batch you are buying. Vitality Supplements publishes batch-specific CoAs and tests every production run.
  • 02
    Verified purity — greater than 99% β-NMN
    NMN should be the β form (beta-NMN) — the biologically relevant isomer studied in published research. Greater than 99% purity is the standard for pharmaceutical-grade raw materials. Lower purity means more impurities, which may include inactive or unwanted compounds. Always ask what the purity figure refers to specifically.
  • 03
    Full dose disclosure — milligrams per ingredient
    Every ingredient should be listed with its exact milligram amount per serving. Avoid "proprietary blends" — these mask the actual dose of each ingredient behind a combined total, making it impossible to assess whether doses align with published research. Full transparency is non-negotiable in a quality supplement.
  • 04
    UK or equivalent manufacturing
    Supplements manufactured in the UK are subject to UK food supplement regulations and food safety law. Manufacturing provenance matters — unregulated or under-regulated manufacturing environments increase the risk of contamination, mislabelling and quality inconsistency. Vitality Supplements products are UK manufactured.
  • 05
    Transparent company with real contact details
    A quality supplement company should publish batch-specific Certificates of Analysis, have a verifiable UK address, respond to enquiries and stand behind their product with a clear returns policy. Anonymous or near-anonymous brands — common on marketplace platforms — offer no accountability. Vitality Supplements provides all of the above, including a 60-day satisfaction guarantee on first purchases.
Red flags to avoid
  • No Certificate of Analysis available, or CoA not batch-specific
  • Purity not stated, or stated without specifying β-NMN isomer
  • Proprietary blends that hide individual ingredient doses
  • Health claims that go beyond what UK food supplement regulations permit
  • Anonymous brands with no verifiable UK address or contact information
  • Extremely low prices that are inconsistent with genuine high-purity raw material costs
  • No returns policy or satisfaction guarantee
Key Research Summary

The research behind the protocol

Systematic Review
NMN and NAD+ — 10 RCTs, 437 participants
A systematic review covering 10 randomised controlled trials with 437 participants documented that oral NMN supplementation consistently elevated blood NAD+ levels across doses from 250 to 900mg per day, with a strong safety profile and no significant adverse events. The highest quality level of published evidence.
Established Research
Resveratrol and SIRT1 — landmark 2003 research
Landmark research published in 2003 documented resveratrol's relationship to SIRT1 activation. This triggered decades of subsequent research into the sirtuin-pathway polyphenol combination with NAD+ precursors. The NMN + resveratrol combination is the most research-supported multi-ingredient longevity formula in terms of published literature.
Pharmacokinetic Research
Pterostilbene — ~4x higher bioavailability than resveratrol
Published pharmacokinetic research documented approximately 4x higher oral bioavailability for pterostilbene compared to resveratrol. This difference — driven by two methoxy groups vs two hydroxyl groups — makes pterostilbene of interest as a higher-bioavailability sirtuin-pathway polyphenol.
Human Research
AHCC — over three decades of published research
AHCC has been the subject of published peer-reviewed research since its development in 1989 in Japan. It has one of the most extensive published research bases of any mushroom-derived supplement ingredient, with studies published across multiple research contexts in cellular biology and immune function research.
FAQ

Common questions

Published human research has used NMN at doses from 250mg to 900mg per day. A systematic review of 10 RCTs with 437 participants documented consistent NAD+ elevation across this range. Vitality Supplements provides 500mg per serving in NMN + Trans-Resveratrol 1100mg and 750mg per serving in NMN Complete 1350mg. As a food supplement, NMN has no authorised recommended daily amount under UK regulations.
TMG (Trimethylglycine) is a methyl donor compound found in beetroot. NAD+ metabolism consumes methyl groups, and some researchers recommend TMG supplementation to support methylation capacity when taking NMN. Published research has documented TMG's role as a methyl donor and its involvement in homocysteine metabolism. Vitality Supplements provides TMG at 750mg per serving in NMN Complete 1350mg.
NMN Complete 1350mg contains four ingredients: 750mg NMN, 750mg TMG, 50mg pterostilbene and 50mg apigenin. It addresses NAD+ precursor, methylation support, sirtuin pathway and CD38 pathway. NMN + Trans-Resveratrol 1100mg contains 500mg NMN and 600mg trans-resveratrol (98% trans-isomer). It addresses NAD+ precursor and sirtuin pathway at a lower price point with a higher resveratrol dose. NMN Complete is more comprehensive. NMN + Resveratrol is the higher-resveratrol, lower-cost starting point.
Five criteria: (1) Independent batch testing by ISO/IEC 17025-accredited laboratory; (2) Greater than 99% β-NMN purity verified; (3) Full dose disclosure — exact milligrams per serving, no proprietary blends; (4) UK or equivalent manufacturing; (5) Transparent company with real contact details and batch-specific CoAs. Read our full NMN buying guide →
Published research has used NMN at various times of day. Morning is the most common timing in published human studies. NAD+ plays a role in circadian rhythm regulation, suggesting morning timing — but this remains active investigation rather than established guidance. Consistency of timing is generally considered more important than the specific time of day. Always follow the serving suggestion on the product label.
Start with the most research-supported NAD+ precursor (NMN). Add a sirtuin-pathway polyphenol (resveratrol or pterostilbene). Consider methylation support (TMG). Address cellular stress biology (AHCC). Prioritise lifestyle — sleep, exercise, diet — as the foundation. Consult a qualified healthcare professional, especially if on medication. Quality matters more than quantity — one well-tested product is worth more than ten poorly-sourced ones.

The complete
Vitality range

NMN Complete 1350mg · NMN + Trans-Resveratrol 1100mg · NMNH 500mg · AHCC 1000mg. UK manufactured. Every batch independently tested by ISO/IEC 17025-accredited laboratory. 60-day satisfaction guarantee on first purchase.

Shop the full range → ← Back to Protocol hub
Pillar 4: Cellular Stress & Inflammaging Back to Protocol hub
Complete the Protocol

This page is part of The Vitality Longevity Protocol — an educational resource covering published peer-reviewed research. Not medical advice. All Vitality Supplements products are food supplements regulated under UK food supplement legislation — not medicines. Not intended to diagnose, treat, cure or prevent any disease. Consult a qualified healthcare professional before starting any supplement. 60-day satisfaction guarantee applies to first purchase of each product only. Contact: info@vitality-supplements.co.uk